Cell-type-specific role of P2Y2 receptor in HDM-driven model of allergic airway inflammation.

Allergic airway inflammation (AAI) is a chronic respiratory disease that is considered a severe restriction in daily life and is accompanied by a constant risk of acute aggravation. It is characterized by IgE-dependent activation of mast cells, infiltration of eosinophils, and activated T-helper cell type 2 (Th2) lymphocytes into airway mucosa. Purinergic receptor signaling is known to play a crucial role in inducing and maintaining allergic airway inflammation. Previous studies in an ovalbumin (OVA)-alum mouse model demonstrated a contribution of the P2Y2 purinergic receptor subtype (P2RY2) in allergic airway inflammation. However, conflicting data concerning the mechanism by which P2RY2 triggers AAI has been reported. Thus, we aimed at elucidating the cell-type-specific role of P2RY2 signaling in house dust mite (HDM)-driven model of allergic airway inflammation. Thereupon, HDM-driven AAI was induced in conditional knockout mice, deficient or intact for P2ry2 in either alveolar epithelial cells, hematopoietic cells, myeloid cells, helper T cells, or dendritic cells. To analyze the functional role of P2RY2 in these mice models, flow cytometry of bronchoalveolar lavage fluid (BALF), cytokine measurement of BALF, invasive lung function measurement, HDM re-stimulation of mediastinal lymph node (MLN) cells, and lung histology were performed. Mice that were subjected to an HDM-based model of allergic airway inflammation resulted in reduced signs of acute airway inflammation including eosinophilia in BALF, peribronchial inflammation, Th2 cytokine production, and bronchial hyperresponsiveness in mice deficient for P2ry2 in alveolar epithelial cells, hematopoietic cells, myeloid cells, or dendritic cells. Furthermore, the migration of bone-marrow-derived dendritic cells and bone-marrow-derived monocytes, both deficient in P2ry2, towards ATP was impaired. Additionally, we found reduced levels of MCP-1/CCL2 and IL-8 homologues in the BALF of mice deficient in P2ry2 in myeloid cells and lower concentrations of IL-33 in the lung tissue of mice deficient in P2ry2 in alveolar epithelial cells. In summary, our results show that P2RY2 contributes to HDM-induced airway inflammation by mediating proinflammatory cytokine production in airway epithelial cells, monocytes, and dendritic cells and drives the recruitment of lung dendritic cells and monocytes.

Protecting against brain damage by improving treatment in neonates with hypoglycaemia: ProBrain-D-a study protocol of a prospective longitudinal study.

INTRODUCTION: Although neonatal hypoglycaemia is the most common metabolic problem in neonates, there is no standard guideline for screening. Additionally, treatment of neonatal hypoglycaemia and glucose administration thresholds are discussed controversially. Severe hypoglycaemia can lead to brain damage, but data on the effects of mild hypoglycaemia on neurological development are limited. To our knowledge, this is the first prospective longitudinal cohort study to analyse if the implementation of a new diagnosis and treatment standard for neonatal hypoglycaemia may improve the outcome of neonates at risk for hypoglycaemia, especially concerning neurodevelopment. Furthermore, the acceptance and feasibility of the standard among different professional groups and parents are analysed. METHODS AND ANALYSIS: After implementation of a structured standard operating procedure (SOP), detailing preventive measures, blood glucose screening and neonatal hypoglycaemia treatment in a tertiary care hospital, 678 neonates ≥35+0 weeks of gestation will be recruited in a monocentric prospective cohort study. For comparison, 139 children born before the implementation of this new SOP, who had risk factors for neonatal hypoglycaemia or qualified for blood glucose measurements are recruited (retrospective cohort). For the primary end point, comparative analyses between and within the prospective and retrospective cohorts will be performed regarding the neurological outcome at 2-2.5 years of age in Bayley Scales of Infant Development. Furthermore, comprehensive clinical data and data on nutrition and developmental milestones are assessed at different time points (6 weeks, 6, 12, 18 and 24 months) in the prospective cohort. Acceptance and feasibility of the new standard are assessed using questionnaires. ETHICS AND DISSEMINATION: The study has been approved by the Ethics Committee of the Medical Faculty of the Heinrich-Heine-University Düsseldorf (20201162). The results of this study will be disseminated through peer-reviewed journals and presented at international conferences. TRIAL REGISTRATION NUMBER: DRKS00024086.

Spontaneous emission of matter waves from a tunable open quantum system.

The decay of an excited atom undergoing spontaneous photon emission into the fluctuating quantum-electrodynamic vacuum is an emblematic  example of the dynamics of an open quantum system. Recent experiments have demonstrated that the gapped photon dispersion in periodic structures, which prevents photons in certain frequency ranges from propagating, can give rise to unusual spontaneous-decay behaviour, including the formation of dissipative bound states1-3. So far, these effects have been restricted to the optical domain. Here we demonstrate similar behaviour in a system of artificial emitters, realized using ultracold atoms in an optical lattice, which decay by emitting matter-wave, rather than optical, radiation into free space. By controlling vacuum coupling and the excitation energy, we directly observe exponential and partly reversible non-Markovian dynamics and detect a tunable bound state that contains evanescent matter waves. Our system provides a flexible platform for simulating open-system quantum electrodynamics and for studying dissipative many-body physics with ultracold atoms4-6.

In situ magnetometry for experiments with atomic quantum gases.

Precise control of magnetic fields is a frequent challenge encountered in experiments with atomic quantum gases. Here we present a simple method for performing in situ monitoring of magnetic fields that can readily be implemented in any quantum-gas apparatus in which a dedicated field-stabilization approach is not feasible. The method, which works by sampling several Rabi resonances between magnetically field sensitive internal states that are not otherwise used in a given experiment, can be integrated with standard measurement sequences at arbitrary fields. For a condensate of 87Rb atoms, we demonstrate the reconstruction of Gauss-level bias fields with an accuracy of tens of microgauss and with millisecond time resolution. We test the performance of the method using measurements of slow resonant Rabi oscillations on a magnetic-field sensitive transition and give an example for its use in experiments with state-selective optical potentials.

Evidence for a quantum-to-classical transition in a pair of coupled quantum rotors.

The understanding of how classical dynamics can emerge in closed quantum systems is a problem of fundamental importance. Remarkably, while classical behavior usually arises from coupling to thermal fluctuations or random spectral noise, it may also be an innate property of certain isolated, periodically driven quantum systems. Here, we experimentally realize the simplest such system, consisting of two coupled, kicked quantum rotors, by subjecting a coherent atomic matter wave to two periodically pulsed, incommensurate optical lattices. Momentum transport in this system is found to be radically different from that in a single kicked rotor, with a breakdown of dynamical localization and the emergence of classical diffusion. Our observation, which confirms a long-standing prediction for many-dimensional quantum-chaotic systems, sheds new light on the quantum-classical correspondence.

Glassy behavior in a binary atomic mixture.

We experimentally study one-dimensional, lattice-modulated Bose gases in the presence of an uncorrelated disorder potential formed by localized impurity atoms, and compare to the case of correlated quasidisorder formed by an incommensurate lattice. While the effects of the two disorder realizations are comparable deeply in the strongly interacting regime, both showing signatures of Bose-glass formation, we find a dramatic difference near the superfluid-to-insulator transition. In this transition region, we observe that random, uncorrelated disorder leads to a shift of the critical lattice depth for the breakdown of transport as opposed to the case of correlated quasidisorder, where no such shift is seen. Our findings, which are consistent with recent predictions for interacting bosons in one dimension, illustrate the important role of correlations in disordered atomic systems.

Collinear four-wave mixing of two-component matter waves.

We demonstrate atomic four-wave mixing of two-component matter waves in a collinear geometry. Starting from a single-species Bose-Einstein condensate, seed and pump modes are prepared through microwave state transfer and state-selective Kapitza-Dirac diffraction. Four-wave mixing then populates the initially empty output modes. Simulations based on a coupled-mode expansion of the Gross-Pitaevskii equation are in very good agreement with the experimental data. We show that four-wave mixing can play an important role in studies of bosonic mixtures in optical lattices. Moreover, our system should be of interest in the context of quantum atom optics.

Superfluidity of interacting bosonic mixtures in optical lattices.

We report the observation of many-body interaction effects for a homonuclear bosonic mixture in a three-dimensional optical lattice with variable state dependence along one axis. Near the superfluid-to-Mott insulator transition for one component, we find that the presence of a second component can reduce the apparent superfluid coherence, most significantly when the second component either experiences a strongly localizing lattice potential or none at all. We examine this effect by varying the relative populations and lattice depths, and discuss the observed behavior in view of recent proposals for atomic-disorder and polaron-induced localization.

Analysis of Kapitza-Dirac diffraction patterns beyond the Raman-Nath regime.

We study Kapitza-Dirac diffraction of a Bose-Einstein condensate from a standing light wave for a square pulse with variable pulse length but constant pulse area. We find that for sufficiently weak pulses, the usual analytical short-pulse prediction for the Raman-Nath regime continues to hold for longer times, albeit with a reduction of the apparent modulation depth of the standing wave. We quantitatively relate this effect to the Fourier width of the pulse, and draw analogies to the Rabi dynamics of a coupled two-state system. Our findings, combined with numerical modeling for stronger pulses, are of practical interest for the calibration of optical lattices in ultracold atomic systems.

The onset of matter-wave amplification in a superradiant Bose-Einstein condensate.

The interaction of short and strong laser pulses with an atomic Bose-Einstein condensate is found to generate patterns of recoiling atoms that are different from those seen in previous light-scattering experiments. This phenomenon can only be explained by optical stimulation, showing that the previous description of superradiance as atomic stimulation is incomplete and that matter-wave amplification in Bose-Einstein condensates is suppressed at short times. Our experiments clarify the nature of bosonic stimulation in the four-wave mixing of light and atoms.